DESCRIPTION

Peripherin is a neuron-specific intermediate filament protein that is particularly expressed in peripheral nervous system. It is used to study peripheral nerve development and regeneration, as it is highly expressed during elongation of axon. Peripherin is a routine marker for unmyelinated neurons in trigeminal ganglion (TG) and the dorsal root ganglion (DRG). Peripherin is also expressed in peri-insular areas of islets and it has been identified as a key autoantigen for type 1A diabetes. Peripherin is a neuronal intermediate filament associated with inclusion bodies in motor neurons of patients with amyotrophic lateral sclerosis (ALS). Mutations in Peripherin have been associated with susceptibility to ALS.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	964.9	54.1	5.6
2	8	221.7	11.8	5.3
3	8	112.1	7.0	6.2

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	974.5	50.6	5.2
2	16	226.6	12.2	5.4
3	16	108.9	7.7	7.1

RECOVERY

The recovery of human/mouse/rat Peripherin spiked to three different levels throughout the range of the assay in lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Lysate	1:64	89	80-98

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The lysate was initially diluted 1:100.)

		Lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	99
	Range (%)	94-103
1:8	Average% of Expected	101
	Range (%)	95-107
1:16	Average% of Expected	101
	Range (%)	94-108

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human/mouse/rat Peripherin produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)