DESCRIPTION

S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs and has been implicated in the regulation of cellular activities such as metabolism, motility and proliferation. S100B is highly expressed in astrocytes in the brain. It is also expressed in the Schwann cells of the peripheral nervous system, and outside the nervous system in melanocytes, adipocytes and chondrocyte. Elevated S100B levels in cerebrospinal fluid and serum reported after head trauma, subarachnoid hemorrhage, and stroke were correlated with the extent of brain damage. An association between elevated serum S100B and the presence of brain metastases has been reported. Increased S100B levels may also be found in patients with cerebral small vessel disease (SVD).

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	2,066.1	160.4	7.8
2	8	979.2	52.8	5.4
3	8	129.6	12.2	9.4

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	2,209.1	208.6	9.4
2	16	923.8	79.9	8.7
3	16	137.7	14.7	10.6

RECOVERY

The recovery of human/mouse/rat S100B spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Tissue lysate	1:4,000	89	85-94
Human cerebrospinal fluid (CSF)	1:16	109	91-127
	1:32	93	89-96

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The tissue lysate was initially diluted 1:4,000. The human cerebrospinal fluid (CSF) was initially diluted 1:2.)

		Tissue lysate	Human cerebrospinal fluid (CSF)
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	104	109
	Range (%)	102-106	108-110
1:8	Average% of Expected	103	119
	Range (%)	99-107	118-120
1:16	Average% of Expected	110	118
	Range (%)	109-111	106-129

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human/mouse/rat S100B produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)