DESCRIPTION

PLEC (Plectin) is a large protein normally expressed in various tissues including skin, muscle, and brain. It binds to many cytoskeletal proteins including microtubules and intermediate filaments and is involved in the establishment and dynamic modulation of the cytoskeletal network. In normal cells, it plays a crucial role in cytoskeleton network organization. In keratinocytes, plectin is concentrated at the basal surface, where it links intermediate filaments to the cytoplasmic domain of transmembrane glycoproteins such as integrin beta-4. (PMID: 24218614, PMID: 28281696, PMID: 23750011)

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	1,069.8	78.1	7.3
2	20	270.3	13.2	4.9
3	20	80.9	4.7	5.9

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	1,067.7	71.8	6.7
2	24	259.0	14.5	5.6
3	24	86.1	5.2	6.1

RECOVERY

The recovery of human/mouse/rat PLEC spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:32	100	96-105
	1:64	101	93-105
Tissue lysate	1:32	118	108-123

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate samples were initially diluted 1:400. The tissue lysate samples were initially diluted 1:4.)

		Cell lysate	Tissue lysate
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	108	89
	Range (%)	107-109	78-99
1:8	Average% of Expected	118	95
	Range (%)	116-120	75-115
1:16	Average% of Expected	114	-
	Range (%)	112-116	-

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human/mouse/rat PLEC produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)