DESCRIPTION

NOX1 (NADPH Oxidase 1) is a membrane-bound NADPH oxidase belonging to the NOX family. It is primarily localized in the plasma membrane and endoplasmic reticulum membrane, where it catalyzes the oxidation of NADPH to generate superoxide anion (O₂⁻) and hydrogen peroxide (H₂O₂). These reactive oxygen species (ROS) play crucial roles in cell signaling, oxidative stress responses, inflammation, and immune defense. The activity of NOX1 is regulated by several accessory subunits (such as p22phox, NOXO1, NOXA1, and RAC1), which together form a complex to exert its function. Aberrant expression of NOX1 is associated with various diseases, including cardiovascular diseases, inflammatory disorders, and certain types of cancer. Therefore, NOX1 is an important target for studying the mechanisms of oxidative stress, inflammation, and tumorigenesis.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	102.74	8.08	7.86
2	8	21.05	1.80	8.57
3	8	10.34	0.53	5.13

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	103.15	7.50	7.27
2	16	21.14	1.73	8.19
3	16	10.68	0.83	7.78

RECOVERY

The recovery of human/mouse/rat NOX1 spiked to three different levels throughout the range of the assay in lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Lysate	1:8	90	86-93
	1:16	87	85-89

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The lysate was initially diluted 1:5. )

		Lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	110
	Range (%)	108-112
1:8	Average% of Expected	100
	Range (%)	87-114
1:16	Average% of Expected	93
	Range (%)	80-106

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human/mouse/rat NOX1 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)