Aliases:	UBR5; Ubiquitin Protein Ligase E3 Component N-Recognin 5; HYD; EDD; KIAA0896; EDD1; DD5; E3 Ubiquitin-Protein Ligase, HECT Domain-Containing 1; HECT-Type E3 Ubiquitin Transferase UBR5; Hyperplastic Discs Protein Homolog; E3 Ubiquitin-Protein Ligase UBR5; Progestin-Induced Protein; E3 Ubiquitin Protein Ligase, HECT Domain Containing, 1; E3 Identified By Differential Display; EC 2.3.2.26; EC 6.3.2; HHYD
Background:	UniProt Entry: O95071;NCBI Gene Entry: 51366
Application Information	Molecular Weight: Predicted, 309 kDa, observed, 309 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB2540 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM)
Immunogen	A synthesized peptide derived from human EDD
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of UBR5 expression in HT-1080 cells using anti-UBR5 antibody 1:2,000. Green, isotype control; red, UBR5.

Western blotting analysis using anti-UBR5 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-UBR5 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit .