DESCRIPTION

MMP-13 (Matrix metalloproteinase 13), also known as collagenase-3, cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial collagenase (MMP-1). MMP13 is considered to play a role in the tumor cell proliferation and invasion. MMP13 is a potential prognostic marker for colorectal cancer, breast cancer and other diseases (PMID: 20878116, PMID: 19787229).

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with biotin) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with biotin also binds to the analyte. Streptavidin-HRP binds to the biotin. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	928.6	69.5	7.5
2	20	1,630.0	101.7	6.2
3	20	3,808.3	227.1	6.0

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	1,002.6	78.1	7.8
2	24	1,833.0	111.3	6.1
3	24	4,422.9	363.0	8.2

RECOVERY

The recovery of human MMP-13 spiked to three different levels throughout the range of the assay in cell culture supernatant was evaluated.

Sample Type		Average% of Expected	Range (%)
Human plasma	1:2	89	85-91
	1:4	91	82-100
Cell culture supernatant	1:2	86	83-88
	1:4	85	79-92

LINEARITY

To assess the linearity of the assay, three samples were spiked with high concentrations of human MMP-13 in matrices and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Human plasma(Sample Diuent PT1-ac)	Cell culture supernatant (SampleDiuent PT1-df)
1:2	Average% of Expected	84	96
	Range (%)	81-86	70-122
1:4	Average% of Expected	72	99
	Range (%)	71-73	75-122
1:8	Average% of Expected	81	104
	Range (%)	70-93	81-120
1:16	Average% of Expected	89	107
	Range (%)	81-97	93-121

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human MMP-13 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)