DESCRIPTION

MMP-2 (also designated type IV collagenase) cleaves collagen types IV, V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway. It is ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. MMP-2 contributes to myocardial oxidative stress by regulating the activity of GSK3beta.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with biotin) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with biotin also binds to the analyte. Streptavidin-HRP binds to the biotin. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	20	15.24	0.68	4.5
2	20	4.99	0.27	5.4
3	20	0.65	0.07	10.0

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	24	16.01	1.20	7.5
2	24	4.71	0.26	5.4
3	24	0.83	0.07	8.9

RECOVERY

The recovery of human MMP-2 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human plasma	1:50	113	105-122
	1:100	119	104-129
Cell culture supernatant	1:2	103	87-113
	1:4	98	90-115

LINEARITY

To assess the linearity of the assay, three samples were spiked with high concentrations of human MMP-2 in various matrices and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human plasma samples were initially diluted 1:25)

		Human plasma	Cell culture supernatant
1:2	Average% of Expected	103	88
	Range (%)	88-112	81-96
1:4	Average% of Expected	110	101
	Range (%)	104-116	91-113
1:8	Average% of Expected	119	101
	Range (%)	116-123	93-107
1:16	Average% of Expected	120	99
	Range (%)	118-121	86-109

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human MMP-2 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)