DESCRIPTION

Matrix metalloproteinase 1 (MMP1), which is also known as interstitial collagenase and fibroblast collagenase is a member of the matrix metalloproteinases (MMPs) family. Proteins in this family mainly participate in the breakdown of extracellular matrix both in normal physiological processes and disease processes. Several factors contribute to the expression of MMP1, including endogenous factors, such as polymorphisms and epigenetic regulation in the promoter region of MMP1, as well as exogenous factors, such as the tumor microenvironment. A variety of MMP1 promoter genotypic polymorphisms have been reported in various ethnic populations, and their contributions to different disease risks have been explored. In the tumor microenvironment, a variety of inflammatory factors, including interleukin-8 (IL-8), IL-1β and tumor necrosis factor-α (TNF-α), have been reported to induce MMP1 expression in cancer cells. (PMID: 31115512, PMID: 28334049)

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	950.1	24.4	2.6
2	20	205.6	5.2	2.5
3	20	50.3	2.1	4.1

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	947.1	62.1	6.6
2	24	201.9	4.3	2.1
3	24	53.9	4.8	9.0

RECOVERY

The recovery of human MMP-1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:128	92	86-108
	1:256	89	77-101
Human plasma	1:16	87	86-89
	1:32	83	79-85
Cell culture supernatant	1:64	101	72-125
	1:128	106	72-124

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human serum samples were initially diluted 1:16. The human plasma samples were initially diluted 1:2. The cell culture supernatant samples were initially diluted 1:8.)

		Human serum	Human plasma	Cell culture supernatant
1:2	Average% of Expected	100	100	100
	Range (%)	-	-	-
1:4	Average% of Expected	104	109	107
	Range (%)	96-113	101-119	106-108
1:8	Average% of Expected	99	116	104
	Range (%)	85-114	108-121	98-108
1:16	Average% of Expected	96	113	103
	Range (%)	77-121	105-124	85-114

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human MMP-1 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)