DESCRIPTION

MLLT1 (also known as ENL, YEATS1) is a protein that plays a significant role in transcriptional regulation and epigenetic processes. MLLT1 contains a conserved YEATS domain at its N-terminus, which is crucial for its function as an epigenetic reader. The Cterminal region of MLLT1 includes an ANC1 homology domain, which is involved in protein-protein interactions with components of the Super Elongation Complex (SEC) and DOT1L complex. MLLT1 is a multifunctional protein with critical roles in transcriptional regulation and epigenetic processes. Its involvement in leukemia and other cancers makes it an important target for therapeutic development.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	963.0	21.7	2.3
2	8	243.7	11.9	4.9
3	8	75.3	3.4	4.6

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	961.4	44.1	4.6
2	16	239.5	11.5	4.8
3	16	78.3	4.9	6.2

RECOVERY

The recovery of human MLLT1 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:160	95	94-96
	1:320	96	93-100

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:20.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	105
	Range (%)	100-109
1:8	Average% of Expected	103
	Range (%)	99-108
1:16	Average% of Expected	106
	Range (%)	100-112

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human MLLT1 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)