DESCRIPTION

CD45 is a transmembrane glycoprotein, expressed on almost all hematopoietic cells except for mature erythrocytes, and is an essential regulator of T and B cell antigen receptor-mediated activation. CD45 controls immune function by regulating lymphocyte survival, cytokine responses, and TCR signaling. Additionally, the soluble cytoplasmic tail of CD45 is a soluble factor in human plasma under steady-state conditions, which contributes to the maintenance of T-cell quiescence.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	5.10	0.32	6.24
2	8	1.20	0.06	5.30
3	8	0.59	0.01	2.49

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	4.89	0.41	8.38
2	16	1.19	0.07	5.91
3	16	0.60	0.03	5.09

RECOVERY

The recovery of human CD45 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human plasma	1:16	108	98-127
	1:32	112	101-124
Cell lysate	1:160	110	105-114
	1:320	104	99-109

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human plasma was initially diluted 1:4. The cell lysate was initially diluted 1:80.)

		Human plasma	Cell lysate
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	98	104
	Range (%)	94-102	102-106
1:8	Average% of Expected	100	105
	Range (%)	96-103	105-106
1:16	Average% of Expected	87	107
	Range (%)	87-88	104-110

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human CD45 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)