DESCRIPTION

TNFRSF13C, also known as BAFF receptor (BAFF-R) or CD268, is a receptor for B-cell activating factor (BAFF) and a member of the tumor necrosis factor receptor superfamily. This receptor is expressed mainly in B cells and is crucial for B cell survival and maturation. TNFRSF13C is the principal receptor required for BAFF-mediated mature B-cell survival and plays a significant role in regulating the peripheral B-cell population. Additionally, BAFF-R may be a novel treatment target for primary central nervous system lymphoma.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	5.28	0.14	2.65
2	8	1.34	0.11	8.21
3	8	0.73	0.06	8.22

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	5.21	0.18	3.45
2	16	1.39	0.14	10.07
3	16	0.72	0.06	8.33

RECOVERY

The recovery of human TNFRSF13C spiked to three different levels throughout the range of the assay in human plasma was evaluated.

Sample Type		Average% of Expected	Range (%)
Human plasma	1:2	81	77-84

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Human plasma
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	112
	Range (%)	107-116
1:8	Average% of Expected	124
	Range (%)	111-137

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human TNFRSF13C produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)