Quantitative Strain of Cronobacter sakazakii - Basic Information

Recommended Medium	Peptone: 10.0g, Beef Extract Powder: 3.0g, Sodium Chloride: 5.0g, Agar: 15.0g, pH: 7.3±0.1(25℃)
Growth Conditions	30℃； 18-24h； Aerobic
Storage Conditions	-20℃
Morphology	Colony diameter 1-2mm, round, regular margin, opaque, golden yellow on the front, convex center, smooth surface, glossy surface, moist texture, easy to pick, G- (red), rod-shaped, purity: pure
Procedure	① First culture the strain into a bacterial suspension according to the culture conditions; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the suspension according to the standard formula; ③ Multiply the initial concentration by the survival rate to obtain the actual concentration after lyophilization; ④ Dilute the bacterial suspension according to the actual concentration after lyophilization to obtain the target concentration, and then dispense and lyophilize;
Reference Use	GB 4789.40-2016 National Food Safety Standard - Food Microbiological Examination - Examination of Cronobacter spp. (Enterobacter sakazakii)
Sharing Method	Public Welfare Sharing