Escherichia coli Quantitative Strain - Basic Information

Recommended Medium	Peptone: 10.0g, Beef Extract: 3.0g, Sodium Chloride: 5.0g, Agar: 15.0g, pH: 7.3±0.1 (25℃)
Growth Conditions	37℃; 18-24h; Aerobic;
Storage Conditions	-20℃
Morphology	Colony diameter 1-2mm, round, regular edges, opaque, light yellow on the front, yellow on the reverse, convex center, smooth surface, moist texture, no pigment production, G- (red), rod-shaped, purity: pure
Procedure	① First culture the strain into a bacterial suspension according to the culture conditions; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; ③ The initial concentration multiplied by the survival rate gives the actual concentration after lyophilization; ④ Based on the actual concentration after lyophilization, dilute the bacterial suspension to obtain the target concentration, then aliquot and lyophilize;
Reference Use	Research; Analysis and testing. Strains for research and quality control
Sharing Method	Public welfare sharing