Clostridium sporogenes Quantitative Strain - Basic Information

Growth Conditions	37℃, 24-48h, anaerobic
Storage Conditions	-20℃
Morphology	Purity: Pure
Procedure	① First culture the strain into a bacterial suspension according to the culture conditions; ② Measure the absorbance of the bacterial suspension, and calculate the initial concentration of the bacterial suspension according to the standard formula; ③ Multiply the initial concentration by the survival rate to obtain the actual concentration after lyophilization; ④ Dilute the bacterial suspension according to the actual concentration after lyophilization to obtain the target concentration, and then carry out aliquoting and lyophilization;
Reference Use	Research; Analysis and testing. Teaching and scientific research. Strain used for sterilization effect testing of medical devices in the “Technical Standard for Disinfection”, positive control strain in “GB/T 4789.12-2003 Examination of Clostridium botulinum and Botulinum Toxin”, quality control strain in the 2015 edition of the Chinese Pharmacopoeia. Strain specified in the 2020 edition of the Pharmacopoeia—“General Chapter 1100 Biological Examination Methods”, Pharmacopoeia strain, test strain for “Sensitivity Test” of Recommended Medium, test strain for “Method Suitability Test”, test strain for “Recommended Medium Suitability Test and Controlled Bacteria Test Method Suitability Test”
Sharing Method	Public Welfare Sharing

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