Basic Information of Cronobacter sakazakii Chromogenic Plate Count Reference Material GBW(E)091330

Recommended Medium	Peptone: 10.0g, Beef Extract Powder: 3.0g, Sodium Chloride: 5.0g, Agar: 15.0g, pH: 7.3±0.1 (25℃)
Growth Conditions	37℃, aerobic, 18-24h
Storage Conditions	-20℃
Isolation Source	Clinical isolate
Procedure	① First culture the bacteria into a bacterial suspension according to the culture conditions; ② Measure the absorbance of the bacterial suspension, and calculate the initial concentration of the bacterial suspension according to the standard formula; ③ Multiply the initial concentration by the survival rate to obtain the actual concentration after freeze-drying; ④ Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, then aliquot and freeze-dry;
Intended Use	National food safety standard - Food microbiological examination: Cronobacter spp. (Enterobacter sakazakii) examination
Sharing Method	Public welfare sharing

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