Enterobacter sakazakii (Cronobacter spp.) Chromogenic Plate Count Reference Material GBW(E)091331 - Basic Information

Recommended Medium	Peptone: 10.0g, Beef Extract Powder: 3.0g, Sodium Chloride: 5.0g, Agar: 15.0g, pH: 7.3±0.1 (25℃)
Growth Conditions	37℃, aerobic, 18-24h
Storage Conditions	-20℃
Isolation Source	Clinical isolate
Procedure	① First culture the strain into a bacterial suspension according to the culture conditions; ② Measure the absorbance of the bacterial suspension, and calculate the initial concentration of the bacterial suspension according to the standard formula; ③ Multiply the initial concentration by the survival rate to obtain the actual concentration after lyophilization; ④ Dilute the bacterial suspension based on the actual concentration after lyophilization to obtain the target concentration, then aliquot and lyophilize;
Reference Use	National Food Safety Standard - Food Microbiological Examination - Examination of Cronobacter spp. (Enterobacter sakazakii)
Sharing Method	Public welfare sharing