Stbl3 (containing PiggyBac-EF1a-3×HA-EGFP-EF1a-Neo plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24 h; Aerobic
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (mold 30°C, bacteria 37°C), and shake rapidly to dissolve; ② Wipe the outer wall of the cryovial with an alcohol cotton pad for disinfection, then transfer it to a biosafety cabinet for handling; ③ Unscrew the cap, draw out all the dissolved liquid, and spread it onto 2 corresponding antibiotic-resistant plates, approximately 200 μL per plate; ④ Incubate the antibiotic-resistant plates under the specified conditions above for 18-24 h, then observe (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate it into 50 mL of liquid LB antibiotic-resistant Recommended Medium, culture overnight on a shaker at 37°C; ⑥ According to experimental needs, pipette an appropriate amount of the bacterial culture for plasmid extraction;
Sharing Method	Public Welfare Sharing