Stbl3 (containing PiggyBac-CAG-GFP11x7-Puro plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25℃)
Growth Conditions	37℃; 18-24 h; Aerobic
Storage Conditions	-80℃
Procedure	① Thaw the cryovial in a water bath (molds at 30℃, bacteria at 37℃) and shake rapidly to dissolve; ② Wipe the outer wall of the cryovial with an alcohol swab for disinfection, then transfer it to a biosafety cabinet for handling; ③ Unscrew the cap, withdraw all the dissolved liquid, and spread it onto 2 corresponding antibiotic plates, approximately 200 μL per plate; ④ Incubate the antibiotic plates under the specified conditions above for 18-24 h, then observe (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate it into 50 mL of liquid LB antibiotic Recommended Medium, then culture overnight at 37℃ on a shaker; ⑥ According to experimental needs, take an appropriate amount of the bacterial culture for plasmid extraction;
Sharing Method	Public welfare sharing