DH5α (containing pAW016 plasmid) Escherichia coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, Agar: 15.0 g, Chloramphenicol: 50 μg/mL, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24 hours; Aerobic;
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (mold 30°C, bacteria 37°C), and shake rapidly to dissolve; ② Disinfect the outer wall of the cryovial with an alcohol cotton swab, then transfer to a biosafety cabinet for handling; ③ Unscrew the vial cap, aspirate all the dissolved liquid, and inoculate onto 2 corresponding antibiotic-resistant plates, approximately 200 μL per plate; ④ Incubate the antibiotic-resistant plates under the above specified conditions for 18-24 h, then observe (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate into 50 mL of liquid LB antibiotic-resistant Recommended Medium, culture overnight at 37°C on a shaker; ⑥ According to experimental requirements, pipette an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public welfare sharing