DH5α (containing pACYCDuet-Myc-MBP-HA plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, Agar: 15.0 g, Chloramphenicol: 50 μg/mL, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24 hours; Aerobic;
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (mold: 30°C, bacteria: 37°C), and shake rapidly to dissolve; ② Wipe the outside of the cryovial with an alcohol swab for disinfection, then transfer it to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate all the dissolved solution, and inoculate onto 2 corresponding antibiotic-resistant plates, about 200 μL per plate; ④ Incubate the antibiotic-resistant plates under the specified conditions above for 18-24 h, then observe (do not seal or wrap the plates tightly); ⑤ Pick a single colony and inoculate it into 50 mL of liquid LB antibiotic Recommended Medium, and culture overnight at 37°C on a shaker; ⑥ According to experimental needs, take an appropriate amount of the bacterial culture for plasmid extraction;
Sharing Method	Public Welfare Sharing