DH5α (containing pCMV-FLAG-10×His-TEV-MCS-FLAG-10×His-mCherry plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24 hours; Aerobic;
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (30°C for fungi, 37°C for bacteria), shaking rapidly to dissolve; ② Disinfect the outer surface of the cryovial with an alcohol swab, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate the entire dissolved suspension, and inoculate onto 2 plates with the corresponding antibiotic, approximately 200 μL per plate; ④ Incubate the antibiotic plates under the specified conditions for 18-24 hours, then observe (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate into 50 mL liquid LB with the appropriate antibiotic (Recommended Medium), incubate overnight at 37°C on a shaker; ⑥ According to experimental needs, take an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public Welfare Sharing