DH5α (containing pET28a-BRAF(human) plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24 h; Aerobic
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (37°C) and shake rapidly to dissolve; ② Wipe the outer wall of the cryovial with an alcohol swab for disinfection, then transfer to a biosafety cabinet for further handling; ③ Unscrew the cap, aspirate the entire dissolved suspension, and inoculate onto 1-2 agar plates (approximately 200 μL per plate); ④ Spread evenly and incubate under the growth conditions described above.
Sharing Method	Public Welfare Sharing