DH5α (containing pBluescript II-SK(+) plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25°C)
Growth Conditions	37°C； 18-24 hours； Aerobic；
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (37°C) and shake rapidly to dissolve; ② Wipe the exterior of the cryovial with an alcohol cotton ball for disinfection, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate all the dissolved liquid, and dispense onto 1-2 agar plates (approximately 200 μL per plate); ④ Spread evenly and incubate under the growth conditions described above.
Sharing Method	Public Welfare Sharing