Product Name	Recombinant Rabbit Anti-LEF1 Monoclonal Antibody
Cat No.	SLA1283M
Chinese Name	淋巴增强因子-1重组兔单抗
Alias	ECTD1; ECTD17; LEF-1; TCF10; TCF1ALPHA; TCF7L3; LEF1_HUMAN; LEF1; T cell-specific transcription factor 1-alpha (TCF1-alpha); lymphoid enhancer binding factor 1; lymphoid enhancer-binding factor 1
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	9F6
React Species	Human,Mouse,Rat
Applications	WB (1:1000-2000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) Flow-Cyt (1ug/Test) ICC/IF (1:50-200) IP (1:20-50) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	A synthesized peptide derived from human LEF1
Purification	affinity purified by Protein A
Predicted MW.	44 kDa
Observed MW.	50,40 kDa
Subcellular Location	Nucleus
Gene ID	51176
Swiss-Prot	Q9UJU2
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Transcription factor that binds DNA in a sequence-specific manner.Participates in the Wnt signaling pathway.Activates transcription of target genes in the presence of CTNNB1 and EP300.PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with LEF1 monoclonal antibody, unconjugated (SLA1283M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LEF1 Monoclonal Antibody, Unconjugated(SLA1283M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LEF1 Monoclonal Antibody, Unconjugated(SLA1283M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LEF1 Monoclonal Antibody, Unconjugated(SLA1283M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LEF1 Monoclonal Antibody, Unconjugated(SLA1283M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Human Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LEF1 Monoclonal Antibody, Unconjugated(SLA1283M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LEF1 Monoclonal Antibody, Unconjugated(SLA1283M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with LEF1 Monoclonal Antibody, Unconjugated(SLA1283M) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023)and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.