Product Name	Recombinant Rabbit Anti-FOXO3 Monoclonal Antibody
Cat No.	SLA1078M
Chinese Name	叉头蛋白O3A单克隆抗体
Alias	AF6q21; FKHRL1; FKHRL1P2; FOXO2; FOXO3A; 1110048B16Rik; 2010203A17Rik; Fkhr2; FOXO3_HUMAN; FOXO3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO3_MOUSE; forkhead box O3
Host Species	Mouse
Isotype	IgG2a, Kappa
Clonality	Monoclonal
Clone No.	11D3
React Species	Human
Applications	WB (1:1000-5000) IHC-P (1:100-500) IHC-F (1:100-500) IF (1:100-500) ICC/IF (1:50-200) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated synthetic peptide derived from human FOXO3A
Purification	affinity purified by Protein G
Concentration	1mg/ml
Predicted MW.	74 kDa
Observed MW.	100 kDa
Subcellular Location	Nucleus
Gene ID	2309
Swiss-Prot	O43524
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with FOXO3A monoclonal antibody, unconjugated (SLA1078M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Human Prastate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FOXO3A Monoclonal Antibody, Unconjugated (SLA1078M) at 1:100 overnight at 4°C, followed by conjugation to SL40296G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with FOXO3A Monoclonal Antibody, Unconjugated (SLA1078M) at 1:100 overnight at 4°C, followed by conjugation to SL40296G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.