DH5α (containing pET28a-NSP1 (SARS-CoV-2) plasmid) Escherichia coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24 h; Aerobic
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (37°C) with rapid shaking to dissolve; ② Wipe the outside of the cryovial with an alcohol swab for disinfection, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate the entire dissolved solution, and dispense onto 1-2 agar plates (about 200 μL per plate); ④ Spread evenly and incubate under the culture conditions described above.
Sharing Method	Public Welfare Sharing

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