OVCAR-3 Cell-Specific Culture Medium - Basic Information

Medium	OVCAR-3 Cell-Specific Culture Medium: 85% RPMI-1640 + 15% FBS + 0.01mg/ml Human Insulin
Storage Conditions	Store at 2-8°C, valid for one month
Appearance	Clear and transparent
Procedure	① Preparation: Sterilize pipettes, 50mL centrifuge tubes, 500mL serum bottles, and the biosafety cabinet with UV light for 20 minutes; pre-warm the basal medium in a 37°C water bath. ② Formulation: Flame-sterilize the mouths of the basal medium bottle and serum bottle, aseptically pour 15% serum into 85% basal medium, then add human insulin to a final concentration of 0.01mg/ml. ③ Sealing: Seal the culture medium bottle mouth with parafilm, attach the corresponding product label, and store the medium back in a 4°C refrigerator. Wipe down the biosafety cabinet with alcohol, extinguish the flame, and UV irradiate for 30 minutes.