Product Name	Recombinant Rabbit Anti-H2AX Monoclonal Antibody
Cat No.	SLA1050M
Chinese Name	泛素化组蛋白H2A.X (ub-K119)重组兔单抗
Alias	H2A.X; H2A/X; H2AFX; Hist5-2ax; gammaH2ax; RGD1566119; H2AX_HUMAN; H2AX; Histone H2A.X; H2AX_MOUSE; H2A.X variant histone; H2A histone family, member X; H2A histone family member X; H2AX (ubiquityl-K119); H2AXK119Ub; ub-H2AX; ubiquityl-H2AX; H2AX (ubiquityl-Lys119)
Host Species	Rabbit
Isotype	IgG
Clonality	Monoclonal
Clone No.	11G1
React Species	Human,Mouse,Rat
Applications	WB (1:500-2000) IHC-P (1:200-800) IHC-F (1:200-800) IF (1:200-800) ELISA (1:5000-20000) IP (1:50-200) ChIP (1:50-100) Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
Immunogen	KLH conjugated Synthesised Ubiquitylpeptide derived from human H2AX around the Ubiquitylation site of Lys119
Purification	affinity purified by Protein A
Concentration	1mg/ml
Predicted MW.	16 kDa
Observed MW.	23 kDa
Gene ID	3014
Swiss-Prot	P16104
Storage	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Background	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Jul 2008].
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

Validation Images

Figure 1. 25 ug total protein per lane of various lysates (see on figure) probed with H2AX | Histone H2A.X (ub-K119) monoclonal antibody, unconjugated (SLA1050M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.	Figure 2. Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (SLA1050M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.
Figure 3. Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (SLA1050M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.	Figure 4. Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (SLA1050M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.
Figure 5. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (SLA1050M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.	Figure 6. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (SLA1050M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.
Figure 7. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (SLA1050M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.	Figure 8. Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (SLA1050M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.
Figure 9. Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (SLA1050M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.	Figure 10. Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (SLA1050M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.
Figure 11. Paraformaldehyde-fixed, paraffin embedded Mouse Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (SLA1050M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.	Figure 12. Paraformaldehyde-fixed, paraffin embedded Rat Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (SLA1050M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.
Figure 13. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (SLA1050M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.	Figure 14. Paraformaldehyde-fixed, paraffin embedded Rat Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with H2AX | Histone H2A.X (ub-K119) Monoclonal Antibody, Unconjugated (SLA1050M) at 1:200 overnight at 4°C, followed by incubation with SL0295G-HRP and DAB (C-0010) staining.

 

FOR RESEARCH USE ONLY. Not for use in diagnostic or therapeutic procedures.