M-NFS-60 Cell Specialized Medium - Basic Information

Culture Medium	M-NFS-60 Cell Specialized Medium: 90% RPMI-1640 + 10% FBS + 0.05mM β-Mercaptoethanol + 62ng/ml M-CSF
Storage Conditions	Store at 2-8°C, valid for one month
Appearance	Clear and transparent, light red
Procedure	① Preparation: UV-irradiate pipettes, 50mL centrifuge tubes, 100mL serum bottles, and the clean bench for 20 minutes; pre-warm the basal medium in a 37°C constant-temperature water bath. ② Preparation: Flame-sterilize the mouths of the basal medium bottle and serum bottle, then aseptically pour 50mL of serum, 0.05mM β-Mercaptoethanol, and 62ng/ml M-CSF into 450mL of basal medium. ③ Sealing: Seal the culture medium bottle mouth with parafilm, attach the corresponding product label, and return the culture medium to the 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and UV-irradiate for 30 minutes.