A549/TAX Specialized Medium - Basic Information

Medium	F-12K＋10%FBS＋1%P/S＋0.5-1μg/mL Tax
Storage Conditions	Store at 2-8 C, shelf life 1 month
Appearance	Clear and transparent
Procedure	1) Preparation: UV-irradiate the pipette, 50 mL centrifuge tubes, 100 mL serum bottles and clean bench for 20 min; prewarm the basal medium in a 37 C constant-temperature water bath; 2) Preparation: flame-sterilize the mouths of the basal medium bottle and serum bottle, pour 50 mL serum into 450 mL basal medium without touching the rim; 3) Sealing: seal the mouth of the medium bottle with sealing film, apply the corresponding product label, and return the medium to a 4 C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and UV-irradiate for 30 min;