Primary Smooth Muscle Culture System - Basic Information

Culture Medium	Primary Smooth Muscle Culture System: 88% Primary Smooth Muscle Cell Basal Medium + 1% Primary Smooth Muscle Cell Culture Supplement + 10% FBS + 1% Penicillin-Streptomycin
Storage Conditions	Store at 2-8°C, valid for one month
Appearance	Clear and transparent
Procedure	① Preparation: Place pipettes, 50mL centrifuge tubes, 100mL serum bottles in the biosafety cabinet and sterilize with UV light for 20 minutes; pre-warm the basal medium in a 37°C constant temperature water bath; ② Formulation: Flame-sterilize the openings of the basal medium bottle and serum bottle, then pour the 1% Primary Smooth Muscle Cell Culture Supplement + 10% FBS + 1% Penicillin-Streptomycin into 88mL of basal medium without touching the rims; ③ Sealing: Seal the culture medium bottle opening with parafilm, attach the corresponding product label, and store the medium back in the 4°C refrigerator. Wipe the biosafety cabinet with alcohol, extinguish the flame, and UV sterilize for 30 minutes;