HLEC Specialized Medium - Basic Information

Medium	500ml ECM + 50ml FBS + 5ml ECGS + 5ml P/S
Storage Conditions	Store at 2-8 C, shelf life 1 month
Appearance	Clear and transparent
Procedure	1) Preparation: UV-irradiate the pipette, 50 mL centrifuge tubes, 500 mL serum bottles and clean bench for 20 min; prewarm the basal medium in a 37 C constant-temperature water bath; 2) Preparation: flame-sterilize the mouths of the basal medium bottle and serum bottle, pour 50 mL FBS + 5 mL ECGS + 5 mL P/S into 500 mL ECM without touching the rim; 3) Sealing: seal the mouth of the medium bottle with sealing film, apply the corresponding product label, and return the medium to a 4 C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and UV-irradiate for 30 min;