CTLA4Ig-24 Cell-Specific Culture Medium - Basic Information

Culture Medium	90% DMEM-H + 10% FBS + 0.2 mM Proline + 0.001 mM Methotrexate.
Storage Conditions	Store at 2-8°C, valid for one month.
Appearance	Clear and transparent, light red.
Procedure	① Preparation: UV irradiate the pipette, 50 mL centrifuge tube, 500 mL serum bottle, and clean bench for 20 minutes; pre-warm the basal medium in a 37°C water bath. ② Formulation: Flame-sterilize the bottle mouths of the basal medium and serum, then pour 10% FBS, 0.2 mM Proline, and 0.001 mM Methotrexate aseptically into the 90% DMEM-H. ③ Sealing: Seal the culture medium bottle mouth with sealing film, attach the corresponding product label, and return the medium to a 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and UV irradiate for 30 minutes.