SHP-77 Cell Specific Culture Medium - Basic Information

Culture Medium	88% RPMI-1640 + 10% FBS + 1% L-Glutamine + 1% Sodium Pyruvate
Storage Conditions	Store at 2-8°C, valid for one month
Appearance	Clear and transparent
Procedure	① Preparation: Sterilize pipettes, 50mL centrifuge tubes, 100mL serum bottles, and the clean bench with UV light for 20 minutes; pre-warm the basal medium in a 37°C water bath. ② Formulation: Flame-sterilize the mouths of the basal medium bottle and serum bottle, then aseptically pour 50mL of serum, 1% L-glutamine, and 1% sodium pyruvate into 440mL of basal medium. ③ Sealing: Seal the culture medium bottle with parafilm, attach the corresponding product label, and return the medium to a 4°C refrigerator. Wipe the clean bench with alcohol, extinguish the flame, and UV-sterilize for 30 minutes.