MitoSOX Red is a novel fluorescent probe that specifically detects mitochondrial superoxide in live cells. After entering living cells, the probe selectively enters the mitochondria, and is then oxidized by superoxide in the mitochondria, which fluoresces red. The probe is oxidized by superoxide rather than other reactive oxygen species (ROS) and reactive nitrogen species (RNS), so it can be used as a specific detection probe for mitochondrial superoxide. At the same time, the ability of the probe to be oxidized by superoxide can be blocked by superoxide dismutase (SOD) or superoxide scavengers.

 

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