Grade: Biochemistry

Application: A chromogenic reagent for peroxidase and hepatitis B antigen. It is a novel, safe chromogenic reagent. Compared to traditional chromogenic reagents such as benzidine, o-toluidine, and o-phenylenediamine, TMB offers advantages including high detection sensitivity and excellent stability, while also being safe to use. Animal studies and Ames bacterial mutagenicity tests confirm TMB is non-carcinogenic and non-mutagenic. Currently, TMB is progressively replacing the potent carcinogen benzidine and other carcinogenic benzidine derivatives in applications spanning clinical laboratory testing, forensic analysis, criminal investigation, and environmental monitoring. Particularly in clinical biochemistry, TMB has gained widespread adoption as a novel peroxidase substrate in enzyme-linked immunosorbent assays (ELISA) and enzyme immunoassays (EIA).  

 

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