Product Introduction:

Cartilage tissue is composed of cartilage nucleus and cartilage matrix, and cartilage tissue and its surrounding cartilage membrane form cartilage. Cartilage is divided into transparent cartilage, elastic cartilage, and fibrocartilage based on the different cellulose components contained in the matrix. There are many methods for cartilage staining, such as toluidine blue O method, Alcian blue method, safranin O method, etc.
The dyeing principle of the Modified Saffron-O and Fast Green Stain Kit is that the combination of the basophil cartilage and the basic dye Saffron-O is red, and the eosinophilic bone and the acid dye Fast Green is green or blue, which is in sharp contrast with the red cartilage, so as to distinguish the cartilage from bone tissue. Saffron-O staining is approximately proportional to the concentration of anions, which indirectly reflects the content and distribution of proteoglycans in the matrix. When cartilage is damaged, glycoprotein in cartilage will be released to make uneven distribution of matrix components, resulting in light or non staining of Saffron-O. The cartilage matrix stained with Saffron-O can be quantitatively analyzed by image analysis software.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Experiment picture

Paraffin section of mouse knee joint
Cartilage stains varying shades of red; mineralized bone stains green to blue-green; cell nuclei stain violet-blue.

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