Product Introduction:

Hematoxylin-Eosin staining,which also named HE stain,is the most basic method of routine section staining in pathology.Hematoxylin is an alkaline natural dye, which can stain the nucleus. The main component of chromatin in the nucleus is DNA. In the double helix structure of DNA, the phosphate groups on the two nucleotide chains are outward, making the outer side of the double helix of DNA negatively charged and acidic. It is easy to dye with positively charged hematoxylin basic dye by ion bond or hydrogen bond.

Heidenhain’s Iron-Hematoxylin Stain Kit use ammonium ferric sulfate as oxidant and differentiator, and can display different structures according to different differentiation degrees.After dyeing, all components are black or dark gray black. The hematoxylin staining of different tissue structure can be removed by Heidenhain differentiation solution at different speed, and the order of black color removal is: mitochondria, striated muscle, nuclear chromatin.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Experiment picture

Paraffin section of mouse small intestine
Mitochondria, striated muscle, myelin, chromatin, etc., appear grayish-black.

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