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Product Introduction:
Narrowly speaking, connective tissue refers to the three types of fibers: collagen fibers, reticular fibers, and elastic fibers. Collagen fibers are the most widely distributed and abundant type of fiber. Masson trichrome staining is the most classic method in connective tissue staining and an authoritative and classic technical method for collagen fiber staining. The so-called trichrome staining usually refers to staining the cell nucleus and selectively displaying collagen and muscle fibers. The dyeing principle of this method is related to the size of anionic dye molecules and the permeability of tissues: the size of molecules is reflected by their molecular weight. Small molecular weight dye(acid Red 66 or acid fuchsin)are easy to penetrate tissues with dense structures and low permeability, while large molecular weight dye(light green or aniline blue)can only enter tissues with loose structures and high permeability. So after Masson staining, muscle fibers appear red, while collagen fibers appear green or blue, mainly used to distinguish between collagen fibers and muscle fibers.
The characteristics of the Masson's Trichrome Stain Kit include: ◆Stable staining; ◆Short differentiation time, 1-2 seconds; ◆Clear and bright colors; ◆Widely used, suitable for paraffin and frozen sections; ◆The stained slices are stored for a long time and are not easily faded.
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Experiment picture
Paraffin section of mouse kidney
Cell nuclei stain violet-brown; collagen fibers stain blue; muscle fibers stain red; other background elements stain varying shades of pink-blue to red.
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