Typical Data

The following data and curve are for reference only. The experimenter must establish a standard curve based on their own experimental data.

Standard Conc. (pg/mL)	1000	500	250	125	62.5	31.25	15.625	0
OD Value	2.701	1.534	0.793	0.422	0.227	0.133	0.086	0.037
Corrected OD Value	2.664	1.497	0.756	0.385	0.19	0.096	0.049	0

Linearity

High concentration Human OT was spiked into samples and then serially diluted 2-fold, 4-fold, 8-fold, and 16-fold within the range of the standard curve to assess linearity. Recovery rates and average recovery are shown below.

Dilution		Serum (n=4)	Cultured Cells (n=4)
1:2	Recovery Range (%)	93-113	96-105
	Avg. Recovery (%)	98	98
1:4	Recovery Range (%)	98-115	96-110
	Avg. Recovery (%)	104	104
1:8	Recovery Range (%)	101-119	102-115
	Avg. Recovery (%)	105	110
1:16	Recovery Range (%)	109-121	105-120
	Avg. Recovery (%)	113	117

Specificity

This kit specifically detects recombinant Human OT. Other related proteins were prepared at 50 ng/mL in the dilution buffer and tested for cross-reactivity. No significant cross-reactivity was observed.

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