Typical Data

The following data and curve are for reference only. The experimenter must establish a standard curve based on their own experimental data.

Standard Conc. (ng/mL)	300	150	75	37.5	18.75	9.375	4.688	0
OD Value	2.701	1.533	0.795	0.422	0.221	0.131	0.088	0.039
Corrected OD Value	2.662	1.494	0.756	0.383	0.182	0.092	0.049	0

Linearity

High concentration Human SIRT1 was spiked into samples and then serially diluted 2-fold, 4-fold, 8-fold, and 16-fold within the range of the standard curve to assess linearity. Recovery rates and average recovery are shown below.

 

Dilution		Serum (n=4)	Cultured Cells (n=4)
1:2	Recovery Range (%)	93-112	95-109
	Avg. Recovery (%)	99	101
1:4	Recovery Range (%)	98-115	96-113
	Avg. Recovery (%)	102	107
1:8	Recovery Range (%)	103-118	100-117
	Avg. Recovery (%)	105	111
1:16	Recovery Range (%)	109-120	102-122
	Avg. Recovery (%)	113	120

Specificity

This kit specifically detects recombinant Human SIRT1. Other related proteins were prepared at 50 ng/mL in the dilution buffer and tested for cross-reactivity. No significant cross-reactivity was observed.

 

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