Typical Data

The following data and curve are for reference only. The experimenter must establish a standard curve based on their own experimental data.

Standard Conc. (ng/mL)	10	5	2.5	1.25	0.625	0.3125	0.15625	0
OD Value	2.703	1.534	0.792	0.424	0.227	0.131	0.086	0.04
Corrected OD Value	2.663	1.494	0.752	0.384	0.187	0.091	0.046	0

Linearity

High concentration Rat HEPC was spiked into samples and then serially diluted 2-fold, 4-fold, 8-fold, and 16-fold within the range of the standard curve to assess linearity. Recovery rates and average recovery are shown below.

Dilution		Serum (n=4)	Cultured Cells (n=4)
1:2	Recovery Range (%)	94-113	94-107
	Avg. Recovery (%)	99	97
1:4	Recovery Range (%)	100-115	97-111
	Avg. Recovery (%)	101	104
1:8	Recovery Range (%)	102-119	100-117
	Avg. Recovery (%)	107	113
1:16	Recovery Range (%)	107-121	104-121
	Avg. Recovery (%)	110	118

Specificity

This kit specifically detects recombinant Rat HEPC. Other related proteins were prepared at 50 ng/mL in the dilution buffer and tested for cross-reactivity. No significant cross-reactivity was observed.

 

 

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