Typical Data

The following data and curve are for reference only. The experimenter must establish a standard curve based on their own experimental data.

Standard Conc. (pg/mL)	1000	500	250	125	62.5	31.25	0
OD Value	2.703	1.531	0.791	0.424	0.228	0.135	0.04
Corrected OD Value	2.663	1.491	0.751	0.384	0.188	0.095	0

Linearity

High concentration Human GFAP was spiked into samples and then serially diluted 2-fold, 4-fold, 8-fold, and 16-fold within the range of the standard curve to assess linearity. Recovery rates and average recovery are shown below.

Dilution		Serum (n=4)	Cultured Cells (n=4)
1:2	Recovery Range (%)	94-112	91-106
	Avg. Recovery (%)	99	101
1:4	Recovery Range (%)	98-115	96-112
	Avg. Recovery (%)	102	107
1:8	Recovery Range (%)	101-119	102-115
	Avg. Recovery (%)	108	113
1:16	Recovery Range (%)	108-121	106-124
	Avg. Recovery (%)	112	119

Specificity

This kit specifically detects recombinant Human GFAP. Other related proteins were prepared at 50 ng/mL in the dilution buffer and tested for cross-reactivity. No significant cross-reactivity was observed.

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