Aliases:	CHEK2; Checkpoint Kinase 2; CDS1; CHK2; PP1425; RAD53; Serine/Threonine-Protein Kinase Chk2; CHK2 Checkpoint Homolog; Cds1 Homolog; BA444G7; HuCds1; HCds1; CHK2 (Checkpoint, S.Pombe) Homolog; CHK2 Checkpoint Homolog (S. Pombe); Checkpoint-Like Protein CHK2; EC 2.7.11.1; EC 2.7.11 48; HUCDS1; Hucds1; LFS2
Background:	UniProt Entry: O96017;NCBI Gene Entry: 11200
Application Information	Molecular Weight: Predicted, 61 kDa, observed, 62 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB3460 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Chk2
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Checkpoint kinase 2 expression in HepG2 cells using Checkpoint kinase 2 antibody 1:2,000. Green, isotype control; red, Checkpoint kinase 2.

Immunocytochemical staining of HT-1080 cells with Checkpoint kinase 2 antibody 1:1,000. Nuclei were stained blue with DAPI; Checkpoint kinase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Checkpoint kinase 2 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Checkpoint kinase 2 antibody 1:20,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.