Stbl3 (containing pAAV-CMV-mCherry-U6-SagRNA plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18–24 h; Aerobic
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (mold: 30°C, bacteria: 37°C) with rapid shaking to dissolve; ② Disinfect the outer wall of the cryovial with an alcohol swab, then transfer it to a biosafety cabinet for further handling; ③ Unscrew the cap and aspirate the entire dissolved suspension, then spread it onto 2 plates with the corresponding antibiotic, approximately 200 μL per plate; ④ Incubate the antibiotic plates under the specified conditions above for 18–24 h before observation (do not seal or wrap the plates tightly); ⑤ Pick single colonies and inoculate them into 50 mL of liquid LB antibiotic Recommended Medium, then culture overnight at 37°C on a shaker; ⑥ According to experimental requirements, aspirate an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public welfare sharing