Aliases:	CLIC4; Chloride Intracellular Channel 4; P64H1 2; CLIC4L; H1; Intracellular Chloride Ion Channel Protein P64H1; Chloride Intracellular Channel Protein 4; DKFZP566G223; HuH1; Epididymis Secretory Sperm Binding Protein; Chloride Intracellular Channel 4 Like; MTCLIC; HUH1
Background:	UniProt Entry: Q9Y696;NCBI Gene Entry: 25932
Application Information	Molecular Weight: Predicted, 29 kDa, observed, 29 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB3465 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human CLIC4
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Chloride intracellular channel 4 expression in HAP-1 cells using Chloride intracellular channel 4 antibody 1:2,000. Green, isotype control; red, Chloride intracellular channel 4.

Immunocytochemical staining of HAP-1 cells with Chloride intracellular channel 4 antibody 1:1,000. Nuclei were stained blue with DAPI; Chloride intracellular channel 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-Chloride intracellular channel 4 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Chloride intracellular channel 4 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.