Aliases:	DHFR; Dihydrofolate Reductase; DHFR1; EC 1.5.1.3; DHFRP1; DYR
Background:	UniProt Entry: P00374;NCBI Gene Entry: 1719
Application Information	Molecular Weight: Predicted, 21 kDa, observed, 18 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB3575 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human DHFR
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Dihydrofolate reductase expression in HeLa cells using Dihydrofolate reductase antibody 1:2,000. Green, isotype control; red, Dihydrofolate reductase.

Immunocytochemical staining of Hela cells with Dihydrofolate reductase antibody 1:1,000. Nuclei were stained blue with DAPI; Dihydrofolate reductase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Dihydrofolate reductase antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Dihydrofolate reductase antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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