Aliases:	DIAPH1; Diaphanous Related Formin 1; HDIA1; LFHL1; Mammalian Diaphanous Related Formin 1; Protein Diaphanous Homolog 1; MDia1; DFNA1; DRF1; Diaphanous (Drosophila, Homolog) 1; Diaphanous Homolog 1 (Drosophila); Diaphanous-Related Formin-1; SCBMS; MDIA1; DIAP1; DIA1
Background:	UniProt Entry: O60610;NCBI Gene Entry: 1729
Application Information	Molecular Weight: Predicted, 141 kDa, observed, 155 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB1990 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human DIAPH1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of Diaphanous related formin 1 expression in HepG2 cells using Diaphanous related formin 1 antibody 1:2,000. Green, isotype control; red, Diaphanous related formin 1.

Immunocytochemical staining of HepG2 cells with Diaphanous related formin 1 antibody 1:1,000. Nuclei were stained blue with DAPI; Diaphanous related formin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Diaphanous related formin 1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

Western blotting analysis using anti-Diaphanous related formin 1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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