Aliases:	KARS1; Lysyl-TRNA Synthetase 1; KARS2; KARS; Lysyl-TRNA Synthetase; Lysine--TRNA Ligase; Lysine TRNA Ligase; EC 6.1.1.6; DFNB89; LysRS; Deafness, Autosomal Recessive 89; EC 2.7.7.-; KIAA0070; EC 6.1.1; CMTRIB; DEAPLE; LEPID; KRS
Background:	UniProt Entry: Q15046;NCBI Gene Entry: 3735
Application Information	Molecular Weight: Predicted, 68 kDa, observed, 73 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB6130 Species Reactivity: Human, Mouse, Rat Applications Tested: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human LysRS
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Western blotting analysis using anti-lysyl-tRNA synthetase 1 antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively. Image was developed using NaQ™ ECL Substrate Kit 1:1,000. Nuclei were stained blue with DAPI; Lysyl-tRNA synthetase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Flow cytometric analysis of lysyl-tRNA synthetase 1 expression in HepG2 cells using lysyl-tRNA synthetase 1 antibody (KD-10521, 1:2,000). Green, isotype control; red, lysyl-tRNA synthetase 1.

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