Aliases:	Lysine Acetyltransferase 2B; P/CAF; P300/CBP-Associated Factor; PCAF; Spermidine Acetyltransferase KAT2B; Histone Acetyltransferase KAT2B; K(Lysine) Acetyltransferase 2B; Histone Acetyltransferase PCAF; Histone Acetylase PCAF; EC 2.3.1.48; GCN5L; GCN5; CREBBP-Associated Factor; EC 2.3.1.57; CAF
Background:	UniProt Entry: Q92831;NCBI Gene Entry: 8850
Application Information	Molecular Weight: Predicted, 93 kDa, observed, 93 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB910 Spcies Reacnivity: Human Applctanions Tsed: Western Blotting (WB), Flow Cytometry (FCM), Immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PCAF
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000
Protocols	For general and specific antibody protocols please visit our website. Read all instructions before using this product.

Flow cytometric analysis of PCAF expression in HT-1080 cells using PCAF antibody 1:2,000. Green, isotype control; red, PCAF.

Immunocytochemical staining of HT-1080 cells with PCAF antibody 1:1,000. Nuclei were stained blue with DAPI; PCAF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-PCAF antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:50,000 respectively.

Western blotting analysis using anti-PCAF antibody 1:5,000 and HRP-conjugated goat anti-rabbit secondary antibody 1:20,000 respectively.

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